Guangzhou BioKey Healthy Technology Co.Ltd
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Herpes simplex virus 6 (HSV-6) real-time PCR detection kit (lyophilized)
--96 tests/kit
Intended use:
This kit, adopting Real-Time PCR-Fluorescence technology, is used for detection of herpes simplex virus 6 (HSV-6). It can be used for the laboratory diagnosis and monitoring of the herpes simplex virus 6 (HSV-6) infection. The test results are only for clinical reference, not for case confirmation or exclusion.
Principle:
The specific primers and specific fluorescent probes are designed for the nucleic acid conserved region of herpes simplex virus 6 (HSV-6). The PCR reaction solution and real-time fluorescent quantitative PCR detection technology is applied on the fluorescence quantitative PCR instrument to realize the rapid detection of herpes simplex virus 6 (HSV-6) through the change of fluorescence signal. The detection system contains a human internal control (IC) gene, which is used to monitor whether the specimens and the process of nucleic acid extraction is qualified.
Main components:
| No. | Components | BIK-QL-H0014 |
| | PCR Enzyme Mix (Lyophilized) | 96 tests/bottle |
| 2 | HSV-6 Primer-Probe Mix | 100 μL/vial |
| 3 | Enzyme Mix Buffer (5×) | 400 μL/vial |
| 4 | Positive Control | 100 μL/tube |
| 5 | Negative Control | 100 μL/tube |
| 6 | RNase-free water | 1mL/tube |
| 7 | Paraffin oil | 1.5mL/tube*2 |
Note: Do not mix the components from different batches for detection. The Positive Control of HSV-6 and internal control were constructed artificially, and they were not infectious.
Product lyophilization package:
Specimen requirements:
1.Specimen type :
Urethral swabs, cervical swabs or rectal swab.
2.Specimen preservation:
The specimen can be stored for 3 months at -20±5℃ and for a long time below -70℃.
Reaction mix preparation:
Refer to the tables below to prepare the reaction mix:
| 1×volume required | |
| Resuspended master mix | 14 μL |
| HSV-6 Primer & probe | 1 μL |
| Total volume | 15 μL |
※Multiply the numbers according to the number of tests.
Operation details please check from IFU!