Acute Ahpnd Shrimp Disease Test PCR Kit Early Mortality Syndrome Rapid RT PCR Test

Acute hepatopancreatic necrosis syndrome Early mortality syndrome Nucleic Acid PCR kit

Intended use:

This kit is suitable for the specific detection of Acute hepatopancreatic necrosis syndrome Early mortality syndrome (AHPND/EMS) in bait, water samples, seedlings, adult aquatic animals and processed aquatic products.

Principle:

This kit uses the specific fragment of acute hepatopancreatrosis necrosis (AHPND/EMS) to design the primer probe probe, which can specifically bind to a SECTION of DNA template in the middle of the primer amplification region. During the PCR extension process, the exonuclease activity of Taq enzyme cuts the 5 '-terminal fluorophore from the probe and makes it free in the reaction system. Thus, the 3 '-end quenched group was separated and fluorescence was emitted, which was detected by fluorescence PCR instrument to achieve the detection of nucleic acid of Acute hepatopancreatic necrosis syndrome Early mortality syndrome (AHPND/EMS) in a completely closed reaction system.

Main components:

1. Sample type

Shrimp seedlings, finished aquatic animals, water samples, bait, processed aquatic products, etc

2. Sample collection and transportation

Sample collection and transportation shall be carried out in accordance with NY/T 541. Samples shall be transported to the laboratory as soon as possible under refrigerated conditions to avoid repeated freeze-thaw.

3. Sample preservation

It can be stored for 24 hours at 2℃~8℃, and can be stored for a long time at -20℃.

PCR amplification and fluorescence detection (PCR detection area):

Put each reaction tube into the PCR instrument and perform PCR amplification according to the following procedures:

FAM is selected for detection channels.

Product performance index picture:

Product performance index:

1. Accuracy

The positive reference materials of testing enterprises were all positive.

2. Minimum detection limit: the detection limit of this kit is 3copies/μL.

3. Precision: Coefficient of variation (CV,%) of intra-batch precision Ct value is ≤5%.

Operation details please check from IFU!