Guangzhou BioKey Healthy Technology Co.Ltd
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Feline Calici virus (FCV) PCR kits, real time quantiative PCR Fluorescent Probe Kits
Intended use:
This kit is used for the specific detection of feline calicivirus (FCV) in cat eye, nose and mouth secretions.
Principle:
This kit uses RNA as a template and primers as a starting point to synthesize cDNA strands complementary to RNA template under the action of high-efficiency reverse transcriptase. Choose cat goblet virus gene conservative design specific probe, the probe can with primer amplification area in the middle of a specific binding DNA template, in the process of PCR extension, the circumscribed Taq polymerase enzymes from the 5 'end of fluorescent groups on the probe will be cut down, make it free in the reaction system, thereby quenching groups out of the 3' end, glow, The nucleic acid of cat calicivirus was detected by fluorescence PCR in a completely closed reaction system.
Biokey Pet PCR testing solution.pdf
| Component | Specification |
| PCR reaction liquid | 8 tubes |
| Positive control | 1 tube |
| Negative control | 1 tube |
Storage: -20±5℃
After sample preparation, proceeding to PCR thermal cycling below steps:
| step | cycle | termperature | time |
| 1 | 1 | 50℃ | 20min |
| 2 | 1 | 95℃ | 2min30s |
| 3 | 45 | 95℃ | 10s |
| 60℃ | 30s |
Details please check IFU!