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Feline infectious Peritonitis / corona virus RT qPCR test kit , Taq DNA polymerase probe kit
Intended use:
This kit is suitable for the specific detection of feline infectious peritonitis virus (FIPV) and feline coronavirus (FCoV) in cats pleural and abdominal fluid, feces, cerebrospinal fluid and other samples.
Principle:
Under the action of high-efficiency reverse transcriptase, cDNA strands complementary to RNA template were synthesized with PRIMER as starting point and RNA template as template. Selects the feline infectious peritonitis and cat goblet viruses conservative design specific gene probe, the probe can with primer amplification area in the middle of a specific binding DNA template, in the process of PCR extension, the circumscribed Taq polymerase enzymes from the 5 'end of fluorescent groups on the probe will be cut down, make it free in the reaction system, thereby quenching groups out of the 3' end, The nucleic acid of feline infectious peritonitis virus and feline calicivirus can be detected by fluorescence PCR instrument in the completely closed reaction system.
Main components:
| Component | Specification |
| PCR reaction liquid | 8 tubes |
| Positive control | 1 tube |
| Negative control | 1 tube |
Sample requirements:
1. Sample type
Samples of pleural and abdominal fluid, feces, cerebrospinal fluid, etc
2. Sample collection and transportation
Sample collection and transportation shall be carried out in accordance with NY/T 541. Samples shall be transported to the laboratory as soon as possible under refrigerated conditions to avoid repeated freeze-thaw.
3. Sample preservation
It can be stored for 24 hours at 2℃~8℃, and can be stored for a long time at -70℃.
Using Micgene 242/244/244IVD and ABI QuantStudio5:
PCR amplification and fluorescence Detection (PCR detection area)
Put each reaction tube into the PCR instrument and perform PCR amplification according to the following procedures:
| Step | Cycle number | Temperature | Time |
| 1 | 1 | 50℃ | 20min |
| 2 | 1 | 95℃ | 2min30s |
| 3 | 45 | 95℃ | 10s |
| 60℃ | 30s collect fluorescent |
FAM and VIC/HEX are selected for detection channels.
The results of interpretation
Result interpretation
| FAM channel (FIPV) | VIC/HEX channel (FCoV) | Result interpretation | |
| 1 | - | - | Results were reported negative |
| 2 | + | - | Results were reported FIPV positive |
| 3 | - | + | Results were reported FCoV positive |
| 4 | + | + | Results were reported FIPV,FCoV positive |
Note: "+" means positive, "-" means negative
Positive judgment value or reference interval
| Result | Ct Value |
| Positive | Ct≤38 |
| Negative | No Ct value or Ct value=0 |
| Gray area | 38＜Ct≤45 |
Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 45 is considered as positive, and no Ct value or Ct value ≥45 is considered as negative.
Operation details please check from IFU!