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African swine fever virus Nucleic Acid Detection Kit
(PCR-Fluorescent Probe Method)
Intended use:
This kit is used for diagnosis, monitoring and epidemiological investigation of African swine fever (ASFV).
It is suitable for specific detection of African swine fever virus (ASFV) in pig oral swab, blood, spleen,
lymph node, kidney and other tissue samples.
Principle:
This kit to choose conservative African swine fever virus gene fragment design specific probe, the probe can
with primer amplification area in the middle of a specific binding DNA template, in the process of PCR extension,
the circumscribed Taq polymerase enzymes from the 5 'end of fluorescent groups on the probe will be cut down,
make it free in the reaction system, thereby quenching groups out of the 3' end, glow, The nucleic acid of ASF virus
was detected by fluorescence PCR in a completely closed reaction system.
Main components:
| Component | Specification |
| PCR reaction liquid | 48 tubes |
| Positive control | 1 tube |
| Negative control | 1 tube |
The results of interpretation
| Results | Ct Value |
| Positive | Ct≤40 |
| Negative | No Ct value or Ct value=0 |
| Gray area | 40＜Ct≤45 |
Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 45 is
considered as positive, and no Ct value or Ct value ≥45 is considered as negative.
Details please check IFU!